Determination of aconitase activity
Weblevels of aconitase, whereas its overexpression decreases its levels and activity [15, 25] (Fig. 1). This protocol describes the determination of aconitase activity, which can provide an indirect measurement of Lon activity in vivo. … WebDetermination of Aconitase Activity 4.5 ml. of water in each of a series of thick walled 100 X 14 mm. Pyrex test-tubes are brought to 30”.
Determination of aconitase activity
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WebJun 1, 2015 · The effect of pH on aconitase activity was studied in 100 mM tris–HCl buffer containing 40 mM citrate or 4 mM d, l-isocitrate. Determination of K i was performed by plotting the graphs of dependence of 1/v from the concentration of inhibitor [i] at two fixed concentrations of substrate (citrate) (Dixon, 1953). WebFor quantitative determination of aconitase enzyme activity and high-throughput screening assays for aconitase modulator. Key Features. Fast and sensitive. Linear detection range (20 µL sample): 0.5 to 100 U/L for 20 min reaction. Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay.
WebThe aconitase activity of a sample may be determined by the following equation: Aconitase Activity = B Sample Dilution Factor (milliunit/mL) T V B = Amount (nmole) of … WebBuy Sodium monofluoroacetate, 99% (CAS No. 62-74-8) from Smolecule. Molecular Formula: C2H2FO2.Na C2H2FO2.Na CH2FCOONa C2H2FNaO2. Molecular Weight: 100.02 g/mol. Introduction Sodium monofluoroacetate, also known as 1080, is a highly toxic chemical compound that has been categorized as a Schedule 7 poison in Australia and …
Web0.5M sorbitol at the concentration of 200mg/ml. Aconitase activity was determined by increase in absorbance at 340nm in the presence of 5mM citrate, 0.25mM NADP, 4mM MgCl 2, 10unit/ml of NADP-isocitrate dehydroge-nase (Murakami et al., 2006). 2.3. Determination of hydrogen peroxide and superoxide anion Determination of rosmarinic … WebNov 11, 2003 · Determination of Aconitase Protein Levels and Activity in the Tomato Genotypes Having demonstrated that leaves of the Aco-1 plants exhibited lower expression of the aconitase gene, we next turned our attention to determining the effect this had on the protein amount and activity.
WebAlper Sarikaya, Aconitase 4 trans mechanism for the reversible dehydration of citric acid as well. From this determination, Gawron et al. surmised that the alpha-hydrogen of these substrates does not participate in the role of aconitase mechanism as the trans mechanism is nowhere near the proximity of the changing subunit. Additionally, the authors found …
WebJul 1, 2007 · We examined the hydroxyketone-mediated production of reactive oxygen species by determination of the aconitase activity. Aconitase is the most sensitive enzyme to reactive oxygen species, and thus, we can estimate the generation of superoxide radical by the inactivation of the enzyme (Gardner and Fridovich, 1992, Murakami and … knitting pattern right sideWebDetermination of Aconitase Activity: A Substrate of the Mitochondrial Lon Protease Pedro M. Quirós Abstract Mitochondrial aconitase is a reversible enzyme that catalyzes the … knitting pattern scarf easyWebAconitase 2 ( ACO2) encodes the mitochondrial aconitase (ACO2), an enzyme catalyzing interconversion of citrate into isocitrate in the Krebs cycle. ACO2 mutations have been … red diamond accountantsWebJun 23, 2024 · Prior studies indicated that H129 is the only histidine ligand involved in the control of the iron donation capability of YtfE, as its replacement for a leucine residue produces a protein unable to restore the aconitase activity . Interestingly, H129 forms an H-bond with E159 (H129 Nδ1 …E159 Oε1 2.66 Å). red diamond 501 engine specsWebAconitase activity determined after 15 hours incubation at 5', pH 7.4. effect. Phosphate thus competed with the protein for the iron in the system, as well as prevented the added … knitting pattern scarf fishermanWebDetermination of Aconitase Activity—For the determination of the aconitase activity, cells from the CGXII main culture (∼25 ml) were harvested with ∼25 g of crushed ice (precooled to -20 °C) by centrifugation at 4,000 × g for 5 min. The cell pellet was resuspended in 900 μl of Tris-HCl (90 m m g; 4 °C), the supernatant was used ... knitting pattern shawl collar cardiganWebSomething that is curious about aconitase is the fact that it uses iron as the inorganic substituent. Mutanogenic studies on aconitase have found substituting the iron ions with … red diamond 5 carats