WebMay 31, 2024 · Biotin-coupled miRNA capture The biotin-coupled miRNA pull-down assay was performed as described previously by Zheng and colleagues ( 16 ). Briefly, the 3′ end biotinylated miR-RNA mimic or control biotin-RNA (RiboBio) was transfected into SPC-A1 cells at a final concentration of 20 nmol/L for 1 day. WebDec 12, 2024 · Streptavidin coupled to a solid matrix of beads allows the specific capture of biotinylated RNA easily. To demonstrate the suitability of biotinylated cap analogues for the study of protein–RNA interactions we established a combined protocol including ultraviolet (UV) cross-linking, and affinity purification of biotinylated-capped RNA.
The CircRNA-ACAP2/Hsa-miR-21-5p/ Tiam1 Regulatory Feedback …
WebNov 23, 2024 · Biotin-coupled miRNA capture, fluorescence in situ hybridization and luciferase reporter assays were conducted to confirm the relationship between circMMP9 and miR-124. In this study, we screened differentially expressed circRNAs and identified circMMP9 in GBM. We found that circMMP9 acted as an oncogene, was upregulated in … WebIn the biotin-coupled miRNA capture assay, the direct interaction between HIPK3 and miR-194 was verified. While upregulating HIPK3 promoted NSCLC cell proliferation, co-transfection with both HIPK3 and miR-194 abrogated the tumor-promoting effects of HIPK3. Altogether, these data corroborated that HIPK3 and miR-149 exert opposing roles in … great south run 2021 parking
circCHST15 is a novel prognostic biomarker that promotes clear …
WebNov 17, 2024 · In addition, a biotin-coupled miRNA capture assay was performed using a biotin-coupled miR-185-3p probe, followed by qRT-PCR. It was observed a fourfold enrichment of circ_0008494 in the miR … WebJan 15, 2024 · Biotin-coupled circRNA and miRNA capture. Biotin-coupled miRNA and circRNA pull-down assays were performed as described previously [11, 12]. Briefly, 3′ end biotinylated circRNA-ACAP2 (digoxin-5′-TCACACAGGCAGCTTTGGAAGAAG-3′-Digoxin; RiboBio, Guangzhou, China) was transfected into SW480 cells at a final concentration of … WebJul 13, 2024 · 2.13 Biotin-coupled miRNA capture. The biotin-coupled miRNA pull-down assay was carried out as previously described. 17, 18 Briefly, the 3′-end biotinylated miR-RNA mimic or control biotin-RNA (RiboBio) was transfected into SPCA1 cells at a final concentration of 20 nmol/L for 1 day. The biotin-coupled RNA complex was pulled … great south run 2021 photos